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Fig. 7 | Journal of Ovarian Research

Fig. 7

From: MicroRNA-424/503 cluster members regulate bovine granulosa cell proliferation and cell cycle progression by targeting SMAD7 gene through activin signalling pathway

Fig. 7

Effect of the sequence-specific knockdown of ACVR2A in bovine granulosa cells. The mRNA (a) and protein level (e) of ACVR2A in bovine granulosa cells transfected with ACVR2A siRNA or siRNA negative control (NC). The proliferation activity in granulosa cells transfected with ACVR2A siRNA or siRNA negative control (NC) (b). The mRNA (c) and protein level (f) of PCNA, and the mRNA (d) and protein level of (f) of STAR in granulosa cells transfected with ACVR2A siRNA or siRNA negative control (NC). The protein expression level of SMAD2/3 and PSMAD2/3 in granulosa cells transfected with siRNA NC or ACVR2A siRNA (g). Flow cytometric analysis showing the cell cycle distribution (G1/G0, S and G2/M phases) of propidium iodide (PI)-labeled bovine granulosa cells transfected with ACVR2A siRNA (i) or siRNA negative control (NC) (h). Bar graphs showing the percentages of cells in G1/G0, S and G2/M phases in granulosa cells transfected with ACVR2A siRNA or siRNA negative control (NC) (j). The bar graphs indicate the mean ± SEM (**p < 0.01). Numbers above western blots represent relative protein density

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Journal of Ovarian Research

ISSN: 1757-2215

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