Fig. 5
From: S-allylcysteine suppresses ovarian cancer cell proliferation by DNA methylation through DNMT1
Treatment of A2780 cells with SAC or 5-aza-dc for 72 h inhibits DNMT activity and decreases the levels of mRNA and protein expressions of DNMT1, DNMT3a and DNMT3b. a total DNMT activity in nuclear extracts was determined using the DNMT Activity Assay Kit. Data are presented in terms of percentage versus the results using non-SAC-treated controls, which was assigned a value of 100%, and as means ± SD; n = 3. b Quantitative real-time PCR analysis of mRNA levels of DNMT1, DNMT3a and DNMT3b in cells. The results are presented as the expression of the individual mRNA with normalization to β-actin and as means ± SD, n = 3. c The levels of DNMT1, DNMT3a and DNMT3b in cell lysates were determined using western blot analysis after treating the cells with SAC for 72 h. The treatment groups of 5-aza-dc were used as controls