Stratification of ovarian tumor pathology by expression of programmed cell death-1 (PD-1) and PD-ligand- 1 (PD-L1) in ovarian cancer

Table 2 Pathological interpretation of IHC stained tissue

Marker	Scoring criteria based on cells/ high power fields^a
T-PD-1	0 = < 1; 1 = 1–10, 2= > 10–50 and 3= > 50
S-PD-1	0 = < 1, 1 = 1–25; 2= > 25–50 and 3= > 50
PD-L1	0 = < 1, 1= > 1–5, 2= > 5–10 and 3= > 10
CD3	0 = < 5, 1 = 5–15, 2= > 15–25, 3= > 25–40 and 4= > 40
CD8	0 = < 1, 1 = 1–25, 2= > 25–50 and 3= > 50
FoxP3	0 = < 1, 1 = 1–5, 2= > 5–15, 3= > 15–25 and 4= > 25 cells/ hpf

^aBased on the known frequency of each marker in various cancer tissues, each stained section was observed over 7–10 hpf and given an average numerical value to represent the scoring frequency based on the categorization in Table 2. PD-1 was assigned 2 different scores to reflect the observed localization of this molecule in tumor tissue. The percentage of PD-1 stained TILs completely enclosed by tumor epithelial cells was termed tumor-PD-1 (T-PD-1). PD-1 stained lymphocytes in the stromal compartment were classified as stromal-PD-1 (S-PD-1)

ISSN: 1757-2215