Fig. 10From: Innovative multi-protectoral approach increases survival rate after vitrification of ovarian tissue and isolated follicles with improved results in comparison with conventional methodSheep oocytes after the live-dead assay. Oocyte were derived from the follicles which were subsequently mechanically isolated, frozen and warmed/thawed. Viability of oocytes were evaluated in staining by calcein-am and ethidium homodimer-1. Letter “a” shows alive oocyte after freezing/thawing showed, “b” - degenerated oocyteBack to article page