Fig. 2

The scheme illustrating “in drop” system for in-vitro culture of isolated follicles. The scheme demonstrates V-shaped well with the 10 μL oil drop on the bottom with 70 μL drop of the culture medium, covered all together in approximately 80 μL of the oil. Additional small oil drop on the bottom ensured that follies were not attachment to the surface of the dish and were suspended in the drop of culture medium, which helped to maintain their 3D structure