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Fig. 1 | Journal of Ovarian Research

Fig. 1

From: Characterization of stem cell and cancer stem cell populations in ovary and ovarian tumors

Fig. 1

Immunolocalization of CD117/C-KIT in normal ovarian and tumor tissue sections: [A] Immunohistochemical detection of Anti-C-KIT polyclonal antibody in OSE (A, B) and ovarian cortex (C, D). Region between dotted boxes in A, C is magnified in B, D respectively. Diffused, faint signals were localized to OSE layer of NO and BN, BL and HG ovarian tumor tissue. C-KIT+ cells appear as single isolated or as clusters in cortex region across all tissue sections. More C-KIT+ immuno-stained cells per field were noticed in BL and HG than NO and BN. Insets denote individual spherical and elongated/spindle shaped cells at higher magnification from other fields of focus. Scale bar = 100 μm (A, C) and 25 μm (B, D) respectively. [B] Immunofluorescence staining of CD117/C-KIT in OSE layer (A, B) and cortex (C) both reveal specific CD117+ cells consistently in NO, BN as well as BL and HG ovarian cancer tissue, where these CD117+ cell numbers are higher in BL OSE and HG cortex per field of focus, compared to NO and BN. White scale bar = 50 μm; blue scale bar = 10 μm. Secondary antibody was conjugated with Alexa fluor 568 and sections were counterstained with nucleus specific dye DAPI

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