Fig. 4

Dual labelling of High Grade (HG) ovarian tumor with OCT4 and KI67: Panel comprises of (a-d) OSE layer and (e-h) cortex [C] of which (a, e) OCT4, Alexa fluor 488, (b, f) KI67, Alexa fluor 568, (c, g) DAPI and (d, h) were merge composites. Both OSE layer (a-d) and cortex (e-f) regions of HG ovary revealed OCT4⁺/KI67⁺ cells. Cortex comprised of cells expressing nuclear OCT4⁺/KI67⁺ and cytoplasmic KI67⁺ (blue asterisk). Few OCT4⁺/KI67⁻ cells (white asterisk) were also observed in the cortex. Tiny spherical (VSELs-like) stem cells were indicated by yellow dotted square. Blue asterisk in (f) denotes cytoplasmic KI67. White scale bar = 50 μm, blue scale bar = 10 μm, green scale bar = 5 μm