Fig. 5

Co-localization of SSEA4 and KI67 in High Grade (HG) ovarian tumor: Panel comprises of (a-d) OSE layer and (e-h) cortex [C] of which (a, e) SSEA4, Alexa fluor 488, (b, f) KI67, Alexa fluor 568, (c, g) DAPI and (d, h) were merge composites. OSE layer prominently showed SSEA4⁺ cells that were KI67⁺ (nuclear and cytoplasmic). In some fields SSEA4⁻/KI67⁺ cells were also observed in cortex. Cytoplasmic SSEA4⁺/KI67⁺ were also observed in some fields of focus. Insets denote representative cells from same field of focus compiled in one panel. Tiny spherical (VSELs-like) stem cells were indicated by yellow dotted square. Blue asterisk denotes cytoplasmic KI67. White scale bar = 50 μm, blue scale bar = 10 μm, green scale bar=5μm (H)