Skip to main content

Table 1 Antibodies used for identification of stem cells, cancer stem cells and proliferating cells from ovaries and ovarian tumor tissue

From: Characterization of stem cell and cancer stem cell populations in ovary and ovarian tumors

Antibody Name

Catalogue Number

Source/Host species

Cross Reactivity

Isotype

Locali-zation

Dilution

Source-Brand

Classification of Markers

ALDH ½

SC-166362

Mouse

Mouse and human

IgG2b

C,N

1/1500

Santa Cruz Biotechn-ology

CSC

CD117/C-KIT

AB-721-SAB4300489

Rabbit

Human

IgG

C

1/250

Sigma

CSC

CD133

MAB4399 Clone 17A6.1

Mouse

Human

IgG2aκ

S

1/100

EMD Millipore

CSC

CD133

NB120–16518

Rabbit

Human, Mouse, and Rat

affinity isolated

S

1/100

Novus Biologicals

CSC/HSC

CD24

SAB1402713-100UG

Mouse

Human

IgG2aκ

S

1/50

Sigma

CSC

CD44-PE/Cy5

103010

Mouse

Mouse, Human

Rat IgG2b, κ

S

1/50

BioLegend

CSC

CD44

SAB1405590

Mouse

Human

affinity isolated

S

1/50

Sigma

CSC

KI67

SAB4501880-100UG

Rabbit

Human

affinity isolated

N,C

1/50

Sigma

PrC

LGR5

MABD148 clone 5G10.1

Mouse

Human

IgG2bκ

S

1/500

Sigma

SC/CSC

OCT-4

MAB4419A4

Mouse

Human and Mouse

IgG1

N,C

1/250

Sigma

SC

SSEA-4

MAB4304

Mouse

Human and Mouse

IgG3

S

1/50

EMD Millipore

SC

  1. CSC Cancer Stem Cell Marker, HSC Hematopoietic Stem Cell Marker, SC Stem Cell Marker, PrC Proliferation Cell Marker, C Cytoplasmic marker, S Surface marker, N Nuclear marker