Table 1 Details of primers used for various experiments
Gene Names | Primer Sequence | Annealing Temp (°C) | Amplicon Size (bp) |
|---|---|---|---|
Pcna | F:GATGCCGTCGGGTGAATTTG R:TCTCTATGGTTACCGCCTCCT | 55 | 182 |
Oct-4 | F:CCTGGGCGTTCTCTTTGGAAAGGTG R: GCCTGCACCAGGGTCTCCGA | 62 | 177 |
Oct-4A | F: CCATGTCCGCCCGCATACGA R: GGGCTTTCATGTCCTGGGACTCCT | 60 | 190 |
Nanog | F: CAGGAGTTTGAGGGTAGCTC R: CGGTTCATCATGGTACAGTC | 61 | 223 |
Sox-2 | F:GGGGGAAAGTAGTTTGCTGCCTCT R:TGCCGCCGCCGATGATTGTT | 61 | 135 |
FSHR1 | F:CATTCACTGCCCACAACTTTCATC R:TGAGTGTGTAATTGGAACCATTGGT | 65 | 256 |
FSHR3 | F:TCTCCACTGCTGCACTGTTGGGCT R:ATTCAAATACAGGAAATAGAGAAA | 56 | 111 |
Sca-1 | F:AGAGGAAGTTTTATCTGTGCAGCCC R:TCCACAATAACTGCTGCCTCCTGA | 58 | 223 |
Stella | F:ACGCTTTGGATGATACAGACGTCC R:GCGCTTTGAACTTCCCTCCGGA | 59 | 175 |
Fragallis | F:GGGGTGACTGAGCTGGGGGAA R:TGTCCCTAGACTTCACAGAGTAGGC | 61 | 178 |
ER | F:CCTTCTAGACCCTTCAGTCAAGC R:CGAGACCAATCATCAGAATCTCC | 65 | 155 |
PR | F: CCAGCTCACAGCGCTTCTACC R: GAAAGAGGAGCGGCTTCACC | 62 | 198 |
18S | F:GGAGAGGGAGCCTGAGAAAC R: CCTCCAATGGATCCTCGTTA | 60 | 171 |