Skip to main content
Fig. 1 | Journal of Ovarian Research

Fig. 1

From: Exosomal miR-1290 is a potential biomarker of high-grade serous ovarian carcinoma and can discriminate patients from those with malignancies of other histological types

Fig. 1

The collection of exosomes from EOC cell lines and the analyses of exosomal miRNAs. (a) Electron microscopy. Exosomes were immunogold-labeled with anti-CD63 antibody and transmission electron micrographs of isolated exosomes secreted from 2 HGSOC cell lines (HeyA8, upper panel;TYK-nu, middle panel) are shown. Immortalized ovarian surface epithelium (IOSE) cells (bottom panel) were used as a non-malignant control. Scale bar, 100 nm. (b) Venn diagrams showing the relative miRNAs expressed in HeyA8 and TYK-nu vs. IOSEs by exosomal miRNA microarray. Circles with solid lines represent counts of exosomal miRNAs with expression levels increased by > 3.0-fold. Circles with dotted lines represent counts of miRNAs with expression levels increased by > 4.0-fold. (c) Summary of exosomal miRNA microarray. Exosomal miRNAs that were upregulated by more than 4.0-fold in both HeyA8 and TYK-nu vs. IOSEs are listed. (d) qRT-PCR of miR-1290. The relative expression of miR-1290 in HGSOC-derived exosomes is shown. IOSE-derived exosomes were used as a control. Data are the mean ± standard deviation (SD) of 3 experiments. **, P < 0.01

Back to article page

Journal of Ovarian Research

ISSN: 1757-2215

Contact us