Fig. 1
DAXX enhances ascites cell colony formation and migration. a–b DAXX-induced ascites cell proliferation. Ascites cells were seeded into 96-well plates (3,000 cells/well) overnight, and then assessed by an MTT assay. Scale bar, 50 μm. c–d Transwell experiment to determine the migration capability of ascites cells and their derivatives (GFP-DAXX-overexpressing ascites cells). Cells were added to transwells and allowed to migrate for 12 h. Cells at the upper surface of the membrane were removed with cotton swabs, and cells on the bottom surface were stained with hematoxylin and eosin. Scale bar, 100 μm. e–f Colony formation assay for the growth of ascites cells with DAXX overexpression. Colony numbers were counted after 10–14 days. * p < 0.05; ***, p < 0.0001. g DAXX overexpression promotes anchorage-independent growth of ascite cells in vitro. Soft-agar colony-formation assay was performed and the colonies were stained with crystal violet for quantification. h DAXX overexpression promoted cell proliferation in suspension (detachment) conditions. Cells (1 × 105) were plated in 6-well culture dishes and cell number was determined with trypan blue staining. Three replicates were included. The error bars represent s.d. ns (p > 0.05); *** p < 0.001. Student’s t-test was applied