Fig. 1

Metabolism of ovarian cancer cell lines and impact of metabolic inhibitors on adult granulosa tumor cell line (KGN) culture viability. Dynamics of metabolism of ovarian cancer cell lines was analyzed through disruption of both glycolysis and oxidative phosphorylation (OXPHOS). Total cellular ATP content was analyzed following 6-h treatments with glycolysis inhibitor 2-deoxy-d-glucose (2-DG) or OXPHOS inhibitor, oligomycin A (Oligo), where indicated conditions annotated with different letters indicates statistical significance between conditions of at least P < 0.05. a SKOV3 cells had significantly reduced total cellular ATP following treatment with 10 mM 2-DG. b OVCAR3 cells and (c) Kuramochi cells decreased in ATP content following treatment with high doses of both 2-DG and Oligo. d KGN cells had a significant decrease in total cellular ATP levels after treatment with 10 mM 2-DG. e Treatment with 5 mM and 10 mM 2-DG over 24 h significantly decreased KGN culture viability, as measured by MTS assay. f Treatment with oligomycin A for 24 showed no changes in KGN culture viability, whereas treatment with the respiratory chain uncoupler carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone (FCCP; g) significantly decreased culture viability at both 2.5 μM and 5.0 μM after 24 h