Fig. 4
KGN and SKOV3, but not Kuramochi, cells exhibit decreased mitochondrial membrane potential (Δψm) following dual treatment with FCCP and TRAIL. Flow cytometry was used to detect changes in Δψm using the fluorescent dye, JC-1. a Δψm independent JC-1 monomers fluoresce at ~ 525 nm (green) throughout the cell, while Δψm dependent JC-1 polymers form following accumulation within mitochondria and fluoresce at ~ 595 nm (orange-red), allowing quantitation of Δψm as the JC-1 red:JC-1 green ratio, representative plots of KGN culture. b Quantitation of the JC-1 red:JC-1 green ratio in KGN culture exhibited a decreased Δψm across all conditions tested (FCCP: 39 ± 7% relative to vehicle treated cells, with differing letters indicating statistical significance. c, d In SKOV3 cells FCCP treatment did not significantly decrease the JC-1 red:JC-1 green ratio, however single treatment with TRAIL decreased Δψm, and was further decreased following treatment with both FCCP and TRAIL (e, f) In Kuramochi cells, no significant changes in Δψm were observed under the same conditions