Table 1 Metabolic inhibition sensitize KGN cultures to cytotoxicity via TRAIL and cisplatin

Untreated

50 ng/mL

100 ng/mL

FCCP (μM)

Vehicle

1.00 ± 0.01^a

0.83 ± 0.06^a*

0.97 ± 0.04^a

1.0

0.94 ± 0.02^a

0.84 ± 0.06^a,b

0.85 ± 0.04^a,b

2.5

0.74 ± 0.05^b

0.74 ± 0.04^b

0.74 ± 0.04^b

5.0

0.68 ± 0.05^b

0.44 ± 0.02^c

0.49 ± 0.06^c*

ANOVA

2.30E-06

2.82E-06

7.48E-08

Untreated

1 μM

10 μM

FCCP (μM)

Vehicle

1.00 ± 0.10^a

1.21 ± 0.16^a

0.98 ± 0.06^a

1.0

0.93 ± 0.07^a

1.03 ± 0.12^a

0.81 ± 0.03^b

2.5

0.91 ± 0.03^a

0.97 ± 0.16^a

0.79 ± 0.01^b

5.0

0.79 ± 0.03^a

0.89 ± 0.08^a

0.59 ± 0.03^c*

ANOVA

0.1798

0.427

0.0005

Untreated

50 ng/mL

100 ng/mL

Oligomycin (μM)

Vehicle

1.00 ± 0.05^a

1.15 ± 0.08^a

0.96 ± 0.02^a

1.0

0.99 ± 0.08^a

1.01 ± 0.03^a

0.81 ± 0.01^b

2.5

1.00 ± 0.07^a

0.97 ± 0.01^a

0.82 ± 0.02^b*

5.0

1.06 ± 0.02^a

0.97 ± 0.03^a

0.83 ± 0.02^b*

ANOVA

0.8191

0.0827

0.0013

Untreated

1 μM

10 μM

Oligomycin (μM)

Vehicle

1.00 ± 0.05^a

0.85 ± 0.04^a

0.65 ± 0.07^a

1.0

1.08 ± 0.06^a

1.03 ± 0.08^a

0.84 ± 0.02^b

2.5

1.03 ± 0.03^a

1.05 ± 0.07^a

0.83 ± 0.02^b*

5.0

0.98 ± 0.02^a

0.94 ± 0.03^a

0.77 ± 0.06^b*

ANOVA

0.4821

0.161

0.0802