Table 4 FCCP and oligomycin pretreatment sensitize OVCAR3 cultures to cisplatin treatment

Untreated

50 ng/mL

100 ng/mL

ANOVA

FCCP (μM)

Vehicle

1.00 ± 0.12^a

0.12 ± 0.01^a,b,c*

0.07 ± 0.02^a*

0.0001

1.0

0.98 ± 0.09^a

0.21 ± 0.06^a,b*

0.06 ± 0.01^a*

0.0001

2.5

0.77 ± 0.08^a

0.06 ± 0.05^a,b,c*

0.00 ± 0.02^a,b*

0.0001

5.0

0.70 ± 0.03^a

0.01 ± 0.02^a,c*

−0.02 ± 0.01^b*

5.89E-07

ANOVA

0.0929

0.0436

0.0087

Untreated

1 μM

10 μM

ANOVA

FCCP (μM)

Vehicle

1.00 ± 0.07^a

1.16 ± 0.03^a

0.79 ± 0.03^a*

0.0052

1.0

0.84 ± 0.02^a,b

0.89 ± 0.02^b*

0.60 ± 0.01^b*

8.79E-05

2.5

0.70 ± 0.01^b,c

0.79 ± 0.02^b,c*

0.52 ± 0.01^b,c*

7.98E-05

5.0

0.54 ± 0.03^c

0.57 ± 0.02^d

0.40 ± 0.00^d*

0.0015

ANOVA

0.0002

1.22E-06

2.47E-06

Untreated

50 ng/mL

100 ng/mL

ANOVA

Oligomycin (μM)

Vehicle

1.00 ± 0.01^a

0.09 ± 0.02^a*

0.14 ± 0.01^a*

2.90E-08

1.0

0.94 ± 0.03^a

0.01 ± 0.00^a*

0.07 ± 0.00^b*

6.04E-05

2.5

0.89 ± 0.05^a

0.01 ± 0.02^a*

0.08 ± 0.01^b*

3.19E-06

5.0

0.78 ± 0.08^a

0.06 ± 0.02^a*

0.09 ± 0.01^a*

5.10E-05

ANOVA

0.0988

0.0629

0.0091

Untreated

1 μM

10 μM

ANOVA

Oligomycin (μM)

Vehicle

1.00 ± 0.02^a

1.10 ± 0.05^a

0.91 ± 0.03^a*

0.0385

1.0

0.91 ± 0.04^a

1.09 ± 0.03^a*

0.80 ± 0.03^a

0.0031

2.5

0.92 ± 0.02^a

1.08 ± 0..02^a

0.83 ± 0.05^a

0.1875

5.0

0.90 ± 0.05^a

0.99 ± 0.03^a

0.87 ± 0.02^a

0.0864

ANOVA

0.2729

0.1875

0.2136