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Fig. 4 | Journal of Ovarian Research

Fig. 4

From: Overexpression of CCDC69 activates p14ARF/MDM2/p53 pathway and confers cisplatin sensitivity

Fig. 4

CCDC69 regulates p53, MDM2, and p14ARF stability. a. 293 wildtype and 293 CCDC69 overexpressing cells were treated with 100 μg/mL cycloheximide (CHX) for the indicated periods, followed by Western blot using antibody against p53, MDM2, p14ARF, or CCDC69, with GAPDH as the loading control. The relative p53, MDM2, and p14ARF levels were quantified by densitometry analysis (right). b. Ubiquitylation assay revealing a gradual increase in p14ARF ubiquitylation from CCDC69 overexpression 293 cells. IP, immunoprecipitation; IB, immunoblot

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