Table 2 Primer sets used in sodium bisulfite sequencing analysis
From: Aberrant DNA methylation suppresses expression of estrogen receptor 1 (ESR1) in ovarian endometrioma
Name | Forward | Reverse |
|---|---|---|
ESR1_Region I (− 129 to + 279) | F: 5′-GTTGTGTTTGGAGTGATGTTTAAGTT-3′ | R: 5′-CAATAAAACCATCCCAAATACTTTA-3′ |
ESR1_Region II (− 670 to −94) | F: 5′-GGAAGGGTTTATTTATTTTGGGAGTA-3′ | R: 5′-TAACATTAACTTAAACATCACTCC-3′ |
ESR1_Region III (− 1298 to − 731) | F: 5′-TTGGGTGTTTGGGATAGTAATTAAA-3′ | R: 5′-CTTAATCCCATTAAAAATTCTCAT-3′ |
ESR1_Region IV (− 2227 to − 1756) | F: 5′-TAGTTTTTAAGGGTAGGGGTAAAGG-3′ | R: 5′-CAACAATCCTCATCTCCCTACTAAA-3′ |
ESR1_Region V (− 2595 to − 2241) | F: 5′-TATTTATGGAAAGGTTTGTGGGTTT-3′ | R: 5′-TACTTTCTACTACCACCCCAAACAA-3′ |
ESR1_Region VI (− 2981 to − 2573) | F: 5′-AATTGTATAGTGTTTTAGGGTTAGAGA-3′ | R: 5’-ACCCACAAACCTTTCCATAAATAAC-3’ |
ESR2 (− 113 to + 3) | F: 5′-ATTATTTTTGTGGGTGGATTAGGAG-3′ | R: 5′-AACCCCTTCTTCCTTTTAAAAACC-3′ |