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Table 2 Primer sets used in sodium bisulfite sequencing analysis

From: Aberrant DNA methylation suppresses expression of estrogen receptor 1 (ESR1) in ovarian endometrioma

Name Forward Reverse
ESR1_Region I (− 129 to + 279) F: 5′-GTTGTGTTTGGAGTGATGTTTAAGTT-3′ R: 5′-CAATAAAACCATCCCAAATACTTTA-3′
ESR1_Region II (− 670 to −94) F: 5′-GGAAGGGTTTATTTATTTTGGGAGTA-3′ R: 5′-TAACATTAACTTAAACATCACTCC-3′
ESR1_Region III (− 1298 to − 731) F: 5′-TTGGGTGTTTGGGATAGTAATTAAA-3′ R: 5′-CTTAATCCCATTAAAAATTCTCAT-3′
ESR1_Region IV (− 2227 to − 1756) F: 5′-TAGTTTTTAAGGGTAGGGGTAAAGG-3′ R: 5′-CAACAATCCTCATCTCCCTACTAAA-3′
ESR1_Region V (− 2595 to − 2241) F: 5′-TATTTATGGAAAGGTTTGTGGGTTT-3′ R: 5′-TACTTTCTACTACCACCCCAAACAA-3′
ESR1_Region VI (− 2981 to − 2573) F: 5′-AATTGTATAGTGTTTTAGGGTTAGAGA-3′ R: 5’-ACCCACAAACCTTTCCATAAATAAC-3’
ESR2 (− 113 to + 3) F: 5′-ATTATTTTTGTGGGTGGATTAGGAG-3′ R: 5′-AACCCCTTCTTCCTTTTAAAAACC-3′