Fig. 1

Characterization of HAS2-AS1 in human granulosa cells. (a) Expression of HAS2-AS1 in our library [11] of immature (compact, obtained from GV oocyte) and mature (expanded, obtained from MII oocytes) CCs. (b) The in vivo expression of HAS2-AS1 transcripts in CCs of expanded post-ovulatory MII COCs following IVF treatment proved 3.5-fold higher as compared with CCs of compact GV COCs following IVF treatment (p < 0.01). (c) The in vivo expression of HAS2-AS1 transcripts was also studied in granulosa cells of pre-ovulatory (> 17 mm) follicles obtained in the course of in vitro fertilization (IVF). As shown (c), CCs expressed higher levels of HAS2-AS1 transcripts relative to MGCs (3 fold) counterparts (p = 0.03). All data are normalized to b-actin expression, and represent means ± SEM of three independent experiments