Table 3 Oligonucleotide primers used for real-time PCR amplification of cDNA obtained after reverse transcription from adolescent human ovary RNA
From: The ovarian germinal reserve and apoptosis-related proteins in the infant and adolescent human ovary
GENE | PRIMERS (5′ → 3′) F: forward. R: reverse | AMPLIFIED PRODUCT (bp) |
|---|---|---|
BAX (NM_138761.3)* | F: GCATCGGGGACGAACTGG R: GTCCCAAAGTAGGAGAGGA | 307 |
BCL2 (NM_000633.2) | F: GCCTTCTTTGAGTTCGG R: GGGTGATGCAAGCTCC | 250 |
BID (NM_197966.2) | F: CCTTGCTCCGTGATGTCTTTC R: TCCGTTCAGTCCATCCCATTT | 100 |
MCL-1 L (NM_021960.4) | F: TAAGGACAAAACGGGACTGG R: ACCAGCTCCTACTCCAGCAA | 137 |
MCL-1S (NM_182763.2) | F: GAGACGGCCTTCCAAGGA R: ACCAGCTCCTACTCCAGCAA | 112 |
BCL-XL (NM_138578.1) | F:GCAGGTATTGGTGAGTCGGATCGC R: CACAAAAGTATCCCAGCCGCCG | 100 |
FAS (NM_000043.4) | F: CACTATTGCTGGAGTCAG R: CTGAGTCACTAGTAATGTCC | 266 |
FAS-L (NM_000639.1) | F: TCAATGAAACTGGGCTGTACTTT R: AGAGTTCCTCATGTAGACCTTGT | 101 |
CASPASE-3 (NM_004346.3) | F: CCTCTTCCCCCATTCTCAT R: GAGTCCATTGATTCGCTTCC | 119 |
VASA (NM_024415.2) | F: AGAAAGTAGTGATACTCAAGGACCAA R: TGACAGAGATTAGCTTCTTCAAAAGT | 199 |
OCT3/4 A (NM_002701.3) | F: CTCCTGGAGGGCCAGGAATC R: CCACATCGGCCTGTGTATAT | 341 |
OCT3/4 B (NM_203289.4) | F: ATGCATGAGTCAGTGAACAG R: CCACATCGGCCTGTGTATAT | 263 |
Β-actin (NM_001101.3) | F: CTTCCCCTCCATCGTGGG R: GTGGTACGGCCAGAGGCG | 357 |