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Fig. 2 | Journal of Ovarian Research

Fig. 2

From: Mitochondrially targeted p53 or DBD subdomain is superior to wild type p53 in ovarian cancer cells even with strong dominant negative mutant p53

Fig. 2

(a) Microscopy of EGFP-tagged p53-BakMTS, DBD-BakMTS, E-BakMTS, p53-BaxMTS, DBD-BaxMTS, and E-BaxMTS constructs indicates mitochondrial localization in transfected SKOV-3 cells. The first column (from the left; green color) shows EGFP tagged constructs, the 2nd column (red color) shows stained mitochondria. The 3rd column (blue color) shows the nucleus stained with Hoechst, and the last column shows the overlay. (b) The PCC value generated for each individual cell in each construct was graphed. A PCC value of 0.6 and above is considered colocalized with the mitochondria. Statistical analysis was performed using one-way ANOVA with Tukey’s post-test; ***p < 0.001. Error bars represent standard deviations of PCC values from 30 cells per plasmid constructs in 3 independent experiments (n = 30). All constructs with Bak or Bax contain only the MTS from those proteins (and not the entire protein)

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