Fig. 3

(a) Microscopy of EGFP-tagged p53-BakMTS, DBD-BakMTS, p53-BaxMTS, and DBD-BaxMTS constructs indicates mitochondrial localization in transfected Kuramochi cells. The first column (from the left; green color) shows EGFP tagged constructs, the 2nd column (red color) shows stained mitochondria. The 3rd column (blue color) shows the nucleus stained with Hoechst, and the last column shows the overlay of the 3 individual channels. (b) The PCC values of each construct were generated and graphed. A PCC value of 0.6 and above is considered colocalized with the mitochondria. Error bars represent standard deviations of PCC values from 30 cells per plasmid constructs in 3 independent experiments (n = 30). ***p < 0.001