Fig. 4From: Mitochondrially targeted p53 or DBD subdomain is superior to wild type p53 in ovarian cancer cells even with strong dominant negative mutant p53Nuclear transcriptional activity assay was performed to test the ability of all constructs to activate p53-Luc cis-reporter in various ovarian cell lines: (a) SKOV-3 has mutant but non-expressing p53; (b) ID8 has wild type but very low endogenous p53 expression; (c) Kuramochi has dominant negative p53-D281Y mutation; (d) OVCAR-3 expresses dominant negative p53-R248Q mutation. p53-wt was used as the positive control; EGFP was the negative control. “p53-Luc only” was the experimental control with only the reporter gene. Error bars represent standard deviations from triplicates (n = 3). ***p < 0.001Back to article page