Fig. 6

TMRE assay was performed in (a) ID8 and (b) Kuramochi cell lines (24 h post transfection). TMRE detects mitochondrial outer membrane permeabilization, which occurs as a result of Bak and Bax pore formation, a hallmark of intrinsic apoptosis. Error bars represent standard deviations from triplicates (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001