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Fig. 3 | Journal of Ovarian Research

Fig. 3

From: Hypoxia-inducible factor-1α induces CX3CR1 expression and promotes the epithelial to mesenchymal transition (EMT) in ovarian cancer cells

Fig. 3

Cellular localization of HIF-1α and cell growth/proliferation under hypoxia for OVCAR-3, SW 626 and TOV-112D cells. (a) To determine the effect of hypoxia, the OvCa cells were treated with CoCl2 (150 μM), and with HIF-1α inhibitor KC7F2 for 12 h. Subsequently, cells were fixed and washed, followed by staining with primary (HIF-1α), and secondary antibody (Alexa Flor 488). Nuclei were counterstained with DAPI. Green fluorescence indicates HIF-1α, and red designates phalloidin. The stained cells were imaged with a 40X objective. (b) Morphology of 3D cell growth and proliferation with or without inhibitor under hypoxia. The inhibition by KC7F2 confirmed the role of hypoxia in OvCa cells. (c) Quantitative analysis for surface area of OvCa spheroids over a period of 4 days after treatment with or without inhibitor under hypoxia

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