Fig. 10

Effects of melatonin on E2 and P4 generation in PA- or TG-treated mouse granulosa cells. a E2 levels in mouse granulosa cell culture medium after 48 h of culture. b P4 levels in mouse granulosa cell culture medium after 48 h of culture. c Western blot assays for Star, Cyp11a1 and Cyp19a1 expression at 24 h. d Relative Star expression. e Relative Cyp11a1 expression. f Relative Cyp19a1 expression. The protein expression levels were normalized to the β-actin level. CTL: control; TG: 500 nM thapsigargin; MEL: 10 μM melatonin. The statistical analysis results are shown in the bar graphs. Data are presented as the mean ± SEM of three independent experiments. Bars with different letters are significantly different (p < 0.05)