Fig. 6

Anti-angiogenic effect of 2MeOE2 and DHA on CAM a 2MeOE2 was added to a filter paper in increasing concentrations of 1 μM (n = 9), 10 μM (n = 5) and 100 μM (n = 6), and placed on the CAM. Control group (n = 5) was treated with only the solvent, 100% ethanol. After an incubation of 48 h, CAMs were harvested and viewed under the microscope and number of sprouting vessels around the filter paper was counted. One-way ANOVA, error bars: SEM, p < 0.05. b DHA was added to a filter paper in increasing concentration – 1 μM (n = 9), 10 μM (n = 5), 100 μM (n = 6) and 1 mM (n = 8); and placed on the CAM. Control group (n = 10) treated with only the solvent, 100% ethanol. Following an incubation of 48 h, CAMs were harvested and viewed under the microscope and number of sprouting vessels around the filter paper was counted. One-way ANOVA, error bars: SEM, p < 0.05