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Table 2 List of Antibodies

From: Pro-apoptotic and anti-angiogenic actions of 2-methoxyestradiol and docosahexaenoic acid, the biologically derived active compounds from flaxseed diet, in preventing ovarian cancer

Target Protein

Manufacturer

Raised in

Dilution

Application

ER-α

Santa Cruz, 543

Rabbit

1:100

ICC

Caspase-3

Cell signaling technology, 9665S

Rabbit

1:500

WB

Cleaved Caspase-3

Cell signaling technology, 9664S

Rabbit

1:500

WB

p38

Cell signaling technology, 9212S

Rabbit

1:700

WB

phospho-p38

Cell signaling technology, 4511S

Rabbit

1:700

WB

β-Actin

Santa Cruz, 58,673

Mouse

1:1000

WB

cd-31

Abcam, 28,364

Rabbit

1:50

IHC

α-Smooth muscle actin

Santa Cruz, 32,251

Mouse

1:600

IHC

VEGF

Abcam, 46,154

Rabbit

1:400

IHC

VEGFR-2

Santa Cruz, 6251

Mouse

1:400

IHC

NG2

Abcam, 129,051

Rabbit

1:100

IHC