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Table 1 Summary of the final three decellularization protocols and subsequent sterilization procedure that were optimized in this study. Treatments below the dotted line were equal to all protocols

From: Decellularization of the mouse ovary: comparison of different scaffold generation protocols for future ovarian bioengineering

Details

Protocol 1

Protocol 2

Protocol 3

SDS

SDC

SDS + SDC

Detergent 1

0.5% SDS (10 h)

2% SDC (16 h)

0.5% SDS (5 h)

Wash

dH2O (24 h)

dH2O (24 h)

dH2O (15 h)

Detergent 2

2% SDC (8 h)

Wash

dH2O (24 h)

Pre-treatment

PBS (1 h)

Enzyme

40 IU DNase / ml of PBS (30 min; 37 °C)

Wash

PBS (24 h)

Sterilization

0.1% Peracetic acid in normal saline (30 min)

Wash

Sterile PBS (6 × 5 min)

Wash

Sterile PBS (24 h)

Total time

85 h

91 h

103 h

  1. Abbreviations: SDS sodium dodecyl sulfate, SDC sodium deoxychocolate, dH2O deionized water, PBS phosphate buffered saline, h hours, min minutes