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Table 1 Summary of the final three decellularization protocols and subsequent sterilization procedure that were optimized in this study. Treatments below the dotted line were equal to all protocols

From: Decellularization of the mouse ovary: comparison of different scaffold generation protocols for future ovarian bioengineering

Details Protocol 1 Protocol 2 Protocol 3
SDS SDC SDS + SDC
Detergent 1 0.5% SDS (10 h) 2% SDC (16 h) 0.5% SDS (5 h)
Wash dH2O (24 h) dH2O (24 h) dH2O (15 h)
Detergent 2 2% SDC (8 h)
Wash dH2O (24 h)
Pre-treatment PBS (1 h)
Enzyme 40 IU DNase / ml of PBS (30 min; 37 °C)
Wash PBS (24 h)
Sterilization 0.1% Peracetic acid in normal saline (30 min)
Wash Sterile PBS (6 × 5 min)
Wash Sterile PBS (24 h)
Total time 85 h 91 h 103 h
  1. Abbreviations: SDS sodium dodecyl sulfate, SDC sodium deoxychocolate, dH2O deionized water, PBS phosphate buffered saline, h hours, min minutes