Table 1 List of primer sequences for real time PCR
Primers | Forward | Reverse |
|---|---|---|
CA-125 | 5′-GCACAATTCCCCCAACCTCC−3′ | 5′-TGCCTCTGGATGGAGGTCTAA−3′ |
MMP-2 | 5′- AGCTGGCCTAGTGATGATGTT −3′ | 5′- TTCAGCACAAACAGGTTGCAG−3′ |
MMP-9 | 5′-CGCAGACATCGTCATCCAGT−3′ | 5′-GAAATGGGCGTCTCCCTGAA−3′ |
TIMP-1 | 5′GACCAAGATGTATAAAGGGTTCCAA−3′ | 5′GAAGTATCCGCAGACACTCTCCAT−3′ |
TIMP-2 | 5′-AGGCGTTTTGCAATGCAGAT−3′ | 5′TCCAGAGTCCACTTCCTTCTCACT−3′ |
TIMP-3 | 5′-CAGGACGCCTTCTGCAACTC−3′ | 5′-AGCTTCTTCCCCACCACCTT−3′ |
GAPDH | 5′-GCACCACCAACTGCTTAGCA−3′ | 5′-CTTCCACGATACCAAAGTTGTCAT−3′ |