Fig. 1
BIRC2 (cIAP1), BIRC3 and BIRC4 expression in GCTs and KGN. Identification of BIRC2, BIRC3 and BIRC4 expression at mRNA level in KGN and GCTs and evaluation of cIAP1 protein expression in GCTs. (a-d) Expression of cIAP1 in 42 different samples of GCTs were analyzed by immunohistochemistry. (a,d) Results of the evaluation, performed by 6 researchers, indicated strong and homogenous cIAP1 staining in 18.2 tumors (mean). (b,d) 5.8 tumors (mean) showed weak cIAP1 staining. (c,d) Heterogeneous cIAP1 staining was visible in 18.0 tumors (mean). Arrows indicate strongly stained nuclei. (a-c) Negative control (rabbit serum) showed no staining (a, inset). Scale bars correspond to 50 μM. (d) Shown are the mean values and SEM. (e) RT-PCR of three independent GCTs and KGN (primers are described in Table 1). Controls consisted of a no reverse transcription (−RT) sample and a no template (H2O) sample