Inhibitor of apoptosis proteins are potential targets for treatment of granulosa cell tumors – implications from studies in KGN

Table 1 List of oligonucleotide primers used for RT-PCR studies

Target	Sequence (5′ – 3′)		Reference	Product size (bp)
BIRC2	Forward	GAC ATC ATC ATT GCG ACC CAC	NM_001166.4	192
BIRC2	Reverse	TGG TTT CCA AGG TGT GAG TAC T	NM_001166.4	192
BIRC3	Forward	AGA ACA CCT GAG ACA TTT TCC CA	NM_001165.4	202
BIRC3	Reverse	GAC ATC ATC ATC GTT ACC CAC A	NM_001165.4	202
BIRC4	Forward	TGT GGA GGA GGG CTA ACT GA	NM_001167.3	83
BIRC4	Reverse	AGA TAT TTG CAC CCT GGA TAC CA	NM_001167.3	83
TNFα	Forward	ATG AGC ACT GAA AGC ATG ATC C	NM_000594.4	217
TNFα	Reverse	GAG GGC TGA TTA GAG AGA GGT C	NM_000594.4	217
MCP-1	Forward	AGG TGA CTG GGG CAT TGA T	NM_002982.3	109
MCP-1	Reverse	GCC TCC AGC ATG AAA GTC TC	NM_002982.3	109
IL8	Forward	TCT TGG CAG CCT TCC TGA	NM_000584.4	271
IL8	Reverse	GAA TTC TCA GCC CTC TTC	NM_000584.4	271
L19	Forward	AGG CAC ATG GGC ATA GGT AA	NM_000981.3	199
L19	Reverse	CCA TGA GAA TCC GCT TGT TT	NM_000981.3	199
HPRT	Forward	CCT GGC GTC GTG ATT AGT GA	NM_000194.2	163
HPRT	Reverse	GGC CTC CCA TCT CCT TCA TC	NM_000194.2	163
PPIA	Forward	AGA CAA GGT CCC AAA GAC	NM_021130.5	118
PPIA	Reverse	ACC ACC CTG ACA CAT AAA	NM_021130.5	118
TBP	Forward	TGC ACA GGA GCC AAG AGT GAA	NM_003194.5	132
TBP	Reverse	CAC ATC ACA GCT CCC CAC CA	NM_003194.5	132

ISSN: 1757-2215