Fig. 3
From: Pioglitazone suppresses excessive follicular development in murine preantral follicles
Estradiol (E2) concentrations in culture media at days 6 (panel a) and day 13 (panel b). 1) CTRL group, base media plus androgen (DHT) vehicle (100% ethanol) and pioglitazone vehicle (dimethyl sulfoxide; DMSO); 2) DHT group, CTRL media plus 500 ng/mL DHT and DMSO; 3) pioglitazone group, CTRL media plus 5 ng/mL pioglitazone and ethanol; and 4) DHT + pioglitazone group, CTRL media plus 500 ng/mL DHT and 5 ng/mL pioglitazone. Data are expressed mean ± standard errors. *P < 0.05, by one-way ANOVA and Student-Newman-Keuls post-hoc analysis