Table 1 Antibodies reagents used in immunohistochemistry
From: Canine ovarian gonadoblastoma with dysgerminoma overgrowth: a case study and literature review
Marker | Type/Clone | Supplier | Dilution | Antigen unmasking | Incubation period | Positive control | Cells of interest |
|---|---|---|---|---|---|---|---|
c-KIT (CD117) | Polyclonal | Dako | 1:450 | RS/WB | ON | Canine mast cell tumour | Germ cells |
α-inhibin | Monoclonal/R1 | AbDSerotec | 1:50 | RS/WB | ON | Canine ovarian tissue | Sex cord cells |
Pan-cytokeratin | Cocktail AE1/AE3 | Menarini | 1:300 | RS/WB | ON | Canine mammary gland | Epithelial cells |
Vimentin | Monoclonal/V9 | Dako | 1:500 | RS/WB | ON | Canine mammary gland | Non-epithelial cells |
Oestrogen receptor | Monoclonal/6F11 | Novocastra | 1:40 | RS/WB | ON | Canine endometrial tissue | Epithelial cells |
EMA | Monoclonal/E29 | Dako | 1:20 | RS/WB | ON | Canine intestinal tissue | Epithelial cells |
Ki-67 | Monoclonal/MIB-1 | Dako | 1:50 | RS/WB | ON | Canine lymphoma with high PI | Cells in proliferation |