Fig. 2
From: Phospholipase C inhibits apoptosis of porcine primary granulosa cells cultured in vitro
The influence of PLC inhibitor and activator on PLC mRNA and protein expression in porcine granulosa cells in vitro. Data are mean ± S.E.M.of three independent replicates. (A) The PLCB1 mRNA expression treated with 0.5 μM U73122 for the times given. (B) One representative western blot band chart for PLCB1 treated with 0.5 μM U73122 for 4 h. (C) The PLCB1 protein abundance treated with 0.5 μM U73122 for 4 h. (D) The PLCB1 mRNA expression treated with 0.5 μM m3M3FBS for the times given. (E) One representative western blot band chart for PLCB1 treated with 0.5 μM m3M3FBS for 4 h. (F) The PLCB1 protein abundance treated with 0.5 μM m3M3FBS for 4 h. One-way ANOVA was used for genes and independent sample t-test was used for proteins. For each treatment, means without common letters are significantly different (p<0.05) (A and D); means with ** indicate a difference at p<0.01 compared with control (C); means without asterisk are not significantly different (F)