Skip to main content
Fig. 5 | Journal of Ovarian Research

Fig. 5

From: Phospholipase C inhibits apoptosis of porcine primary granulosa cells cultured in vitro

Fig. 5

The effect of U73122 and m-3M3FBS on Ca2+ sensitive proteins (PKCβ, CAMKIIα and CalcineurinA protein) and mRNA expression of downstream genes and CTNNB in porcine granulosa cells. The cells were challenged with 0.5 μM U73122 or m-3M3FBS for 4 h. a One representative western blot band chart for PKCβ, CAMKIIα and calcineurinA treated with U73122. bThe protein abundance of PKCβ, CAMKIIα and calcineurinA treated with U73122. c The effect of U73122 on mRNA expression of downstream genes and CTNNB. d One representative western blot band chart for PKCβ, CAMKIIα and calcineurinA treated by m3M3FBS. e The protein abundance of PKCβ, CAMKIIα and calcineurinA added by m-3M3FBS. f The effect of m3M3FBS on mRNA expression of downstream genes and CTNNB. Data are mean ± S.E.M. of three independent replicates. Independent sample t-test was used. For each treatment, means without asterisk are not significantly different, means with * indicate a difference at p<0.05 compared with control, means with ** indicate a difference at p<0.01 compared with control, and means with *** indicate a difference at p<0.001 compared with control

Back to article page