Table 1 Primer Sequences used in this study
From: Phospholipase C inhibits apoptosis of porcine primary granulosa cells cultured in vitro
Primer | Primer sequence (5′-3′) | Genebank ID | Product size/bp |
|---|---|---|---|
β-Actin | F-5′- ATCAAGATCATCGCGCCTCC-3’ | XM_003124280.4 | 169 |
R-5′- AATGCAACTAACAGTCCGCCT-3’ | |||
PLCB1 | F-5′-TGAGAAGGAGGGCAGCTTTGGA-3’ | XM_013985062.1 | 108 |
R-5′-CAGCGAGGTCTTGCTCAATGGT-3’ | |||
Cdc42 | F-5′- ATGTGGAGTGTTCTGCACTCA-3’ | XM_005656039.2 | 132 |
R-5′-GGCTCTGGAGAGACGTTCAT −3’ | |||
NFATC1 | F-5′-GAAAACCGACGGAGACCTGT-3’ | NM_214161.1 | 172 |
R-5′-TATGACTGGAGCGTTGGCAG-3’ | |||
NFATC2 | F-5′-TCCTACCCCACGGTCATTCA-3’ | NM_001113452.1 | 179 |
R-5′-TGTATCCAGCTAAGGTGTGTGTC −3’ | |||
NFKB | F-5′-GAGGTGCATCTGACGTATTC − 3’ | NM_001048232.1 | 138 |
R-5′- CACATCTCCTGTCACTGCAT-3’ | |||
NLK | F-5′-GCGGCTTACAATGGCGGTACAT-3’ | XM_013981185.1 | 122 |
R-5′-TGAAGATGGTGCTGAGGGTGGT-3’ | |||
CTNNB1 | F-5′-TCGCCTTCACTACGGACTACCA-3’ | NM_214367.1;XM_013981492.1; XM_005669377.2;XM_013981494.1 | 180 |
R-5′-TGATGAGCACGAACCAGCAACT-3’ | |||
BAK1 | F-5′-GACAGAAGTGGGGCAAGATCA-3’ | XM_001928147.3 | 190 |
R-5′-CGCTCCAATCAGCTCCCTCT-3’ | |||
BAX | F-5′-GGCCTCCTCTCCTACTTTGG −3’ | XM_013998624.2; XM_003127290.5 | 103 |
R-5′-CTCAGCCCATCTTCTTCCAG −3’ | |||
BCL2 | F-5′-ATCGCCCTGTGGATGACTGAGT-3’ | XM_003121700.4 | 140 |
R-5′-GCCTTCAGAGACAGCCAGGAGA-3’ | |||
CASP3 | F-5′-ATACACGTACTCATGGCGAAC-3’ | XM_005671704.2 | 102 |
R-5′-TCCAGAGTCCACTGATTTGCT-3’ | |||
CASP8 | F-5′-CCAGGATTTGCCTCCGGTTA −3’ | NM_001031779.2 | 144 |
R-5′-TGTGGGATGTAGTCCAGGCT −3’ | |||
TP53/P53 | F-5′-TCGGCTCTGACTGTACCACCAT −3’ | NM_213824.3 | 155 |
R-5′-GGCACAAACACGCACCTCAAAG-3’ |