Fig. 3

ST6Gal-I expression is driven primarily by the P3 promoter. a Graphical representation of the 3 promoter-specific mRNA isoforms of ST6Gal-I (adapted from Dall’Olio et al. [26]). Shaded region indicates the coding sequence, which begins in Exon II and spans through part of Exon VI. b ST6Gal-I mRNA isoforms were amplified by PCR using isoform-specific primers. The PCR products were resolved on agarose gels, and detected with ethidium bromide. The expected sizes for the PCR products are 284 bp for the H form, 363 bp for the YZ form, and 253 bp for the X form. We also performed PCR with primers for the ST6Gal-I coding region (expected product = 372 bp), along with primers for GAPDH as a control (expected product = 371 bp)