Fig. 4
From: Sox2 promotes expression of the ST6Gal-I glycosyltransferase in ovarian cancer cells
Sox2 binds to multiple regions within the ST6GAL1 gene. a Immunoblot verification of Sox2 immunoprecipitation by the ChIP-validated anti-Sox2 antibody. b Schematic depicting three known Sox2-binding elements within the ST6GAL1 gene (positioned at − 4500, + 1000, and + 68,000 relative to the TSS within the P3 promoter). Primers 1A and 1B were designed to flank the − 4500 site, Primers 2A and 2B flank the + 1000 site, and Primers 3A and 3B flank the + 68,000 site. c ChIP assays conducted in Pa-1 ovarian cancer cells show that Sox2 binds all three of the predicted Sox2-binding elements within ST6GAL1. * denotes p < 0.05