Fig. 6

PTEN was a direct target of miR-552 in ovarian cancer cells. a. A potential target site for miR-552 in the 3′-UTR of human PTEN mRNA, as predicted by the program Targetscan. To disrupt the interaction between miR-552 and PTEN mRNA, the target site was mutated. b. Luciferase reporter assays performed in HO8910 miR-552 mimic or HGSOC miR-552 mimic and their control cells transfected with wild-type or mutant PTEN 3′-UTR constructs. c. The mRNA expression of PTEN was checked in HO8910 miR-552 mimic or HGSOC miR-552 mimic and their control cells by real-time PCR. d. The protein expression of PTEN was checked in HO8910 miR-552 mimic or HGSOC miR-552 mimic and their control cells by western blot. e. Significant correlation was observed between miR-552 and PTEN expression in human OC tissues. f. HO8910 and HGSOC cells were transfected with PTEN siRNA and then checked by western bolt assay. g. HO8910 miR-552 mimic or HGSOC miR-552 mimic and their control cells were transfected PTEN siRNA and then subjected to CCK8 assay. h. HO8910 miR-552 mimic or HGSOC miR-552 mimic and their control cells were transfected PTEN siRNA and then subjected to migration assay