Fig. 4
From: Thy-1 predicts poor prognosis and is associated with self-renewal in ovarian cancer
Thy-1 is associated with increased proliferation and self-renewal. Thy-1 knockdowns were generated in A2780 ovarian cancer cells (a). Thy-1 knockdown correlated with decreasing proliferation capacity under the same conditions in vitro (b), and decreasing self-renewal capacity in a limiting dilution tumor sphere formation assay (c). Knockdowns demonstrated lower expression of stem cell transcription factors NANOG and SOX2 (d). Subsequently, A2780 ovarian cancer cells were sorted via FACS into Thy-1 enriched (Thy-1 HI) and non-enriched (Thy-1 LO) populations and the Thy-1 enriched population demonstrated increased proliferation (e) and self-renewal capacity (f). Significance: * P < 0.05, ** P < 0.01, *** P < 0.0001, NS = Not Significant