Fig. 4

MiR503HG downregulated miR-31-5p through methylation. UWB1.289 and A2780 cells were transfected with miR-31-5p mimic or miR503HG expression vector. Overexpression of miR-31-5p and miR503HG was confirmed by qPCR (a). The effects of the overexpression of miR503HG on miR-31-5p expression were analyzed by qPCR (b). The effects of overexpressing miR-31-5p on miR503HG expression were also evaluated by qPCR (c). MSP was performed to analyze the effects of verexpressing miR503HG on the methylation of miR-31-5p gene (d). Experiments were repeated 3 times and mean values were presented. *, p < 0.05