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Fig. 3 | Journal of Ovarian Research

Fig. 3

From: Neuropeptide Y regulates proliferation and apoptosis in granulosa cells in a follicular stage-dependent manner

Fig. 3

NPY receptor Y5 content of granulosa cells in EA is higher than that in LAF. Granulosa cells of PA, EA and LAF were isolated separately from rats primed with eCG, as described in the methods section. Total protein was extracted and NPY receptors contents were assessed by Western blot (n = 3). Representative Western blot results are shown. NPY receptor Y1 (NPY1R) content of granulosa cells in PA was higher than that in LAF. NPY receptor Y2 (NPY2R) contents of granulosa cells showed no significant difference. NPY receptor Y4 (NPY4R) content of granulosa cells in PA was higher than that of EA and LAF. NPY receptor Y5 (NPY5R) content of granulosa cells in PA and EA was higher than that in LAF. These results suggest that expression of NPY receptor is subtype-specific and may be involved in the regulation of granulosa cell functions in a follicular stage-dependent manner. Each granulosa cell replicate was isolated from at least two rats. Results are expressed as mean ± SEM of three independent replicates and analyzed by one-way ANOVA and Tukey’s multiple comparison. *, p < 0.05 (vs. PA); †, p < 0.05 (vs. EA)

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