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Fig. 1 | Journal of Ovarian Research

Fig. 1

From: Estrogen enhances the proliferation and migration of ovarian cancer cells by activating transient receptor potential channel C3

Fig. 1

E2 increased proliferation and migration in ovarian cancer cells. a The expression levels of ER-α in ovarian cancer cells (SKOV3, HEY and ES2 cells) were detected by Western blot analysis. β-Actin was used as a loading control. b The ovarian cancer cell lines ES2, OVCAR, SKOV3, and HEY were treated with 10 nM, 100 nM or 1 μM E2 for different times (24 h, 48 h or 72 h). Cell growth was detected by an MTT assay (OD490 nm). The ovarian cancer cell line SKOV3((c) and (d)) and HEY ((e) and (f))was treated with 10 nM, 100 nM or 1 μM E2 for 48 h. DMSO was used as a negative control. Cell migration was analyzed by a Transwell migration assay. The experiment was repeated three times

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