Fig. 3

Kallistatin (KAL) inhibited the proliferation of ovarian cancer cells in vitro and in vivo. (a) The effect of kallistatin on ovarian cancer cell proliferation as measured by MTT assays; A2780 and UWB1.289 cells were transfected stably with PCMV-NC and PCMV-KAL. A2780 and OVCAR3 cells were transfected transiently with kallistatin siRNA. (b) Colony formation assays were used to measure the effect of kallistatin on A2780, UWB1.289 and OVCAR3 cell growth. (c) Cell cycle analysis of A2780 and OVCAR3 cells. (d, e) UWB1.289 cells stably transfected with PCMV-NC and PCMV-KAL were injected subcutaneously into nude female mice. The tumour weights in the PCMV-KAL group were significantly decreased compared with those in the control group. *p < 0.05, **p < 0.01, ***p < 0.001