Table 2 Primers for qPCR of CPEB1 and ACTB
From: CPEB1 deletion is not a common explanation for premature ovarian insufficiency in a Chinese cohort
Forward primer | Reverse primer | |
|---|---|---|
Exon 1 | 5′-AGCGGCTCGTAGGAGCTTCAT-3’ | 5′-TTACCAGCGGGAACGCCAT-3’ |
Exon 2 | 5′-GATAAAAGATTGCTGGGACAACC-3’ | 5′-CAGATGCCTACCACGTTCAAGT-3’ |
Exon 3 | 5′-GCTTTTCCCAACCTCTGCG-3’ | 5′-TACCCCAGCCAACTCATTCTC-3’ |
Exon 4 | 5′-AGTTTCCAGCACCCTCAGTTAG-3’ | 5′-CACAATAATCTCCACTCCTCCC-3’ |
Exon 5 | 5′-CTTCGCATTTCTCCACCTCTG-3’ | 5′-TGGTTGGGGAGGGAGTGACT- 3’ |
Exon 6 | 5′-AAGCCACCTGTACCTGGAGTG-3’ | 5′-GCCCCACCCTTCAACTCTTA-3’ |
Exon 7 | 5′-CTTCTGCCATTCTTTTCTGTCTC-3’ | 5′-GTGCCCACCATGTTACCAAC-3’ |
Exon 8 | 5′-CTGTCCGATCCTTGCTTCA-3’ | 5′-CCTGCCTATCCACCTACCAC-3’ |
Exon 9 | 5′-AGGGCGTTAGCTTAGCTTCAG-3’ | 5′-GGCATACACCACTCCACCAA-3’ |
Exon 10 | 5′-CCAACGGAGTTACCTGAAAGC-3’ | 5′-CGTACAAAGACCAAGCCCAC-3’ |
Exon 11 | 5′-TGAATCCCAGAGGCATCCAG-3’ | 5′-ACAGAAGAAAGGACCAGGCT-3’ |
Exon 12 | 5′-TGTAACAAGGATGGTGGGTTTG-3’ | 5′-GTTTGGAGAAGGGTGGGAGAC-3’ |
ACTB | 5′-ATTCCTATGTGGGCGACGA-3’ | 5′-TGTGGTGCCAGATTTTCTCC-3’ |