Table 2 PCR primers used for CXCL1 gene sequence analysis
From: Associations of CXCL1 gene 5’UTR variations with ovarian cancer
Exon | Forward primer | Reverse primer | Size (bp) | Tm (°C) |
|---|---|---|---|---|
Exon1 | GCGGGCTGCATCAGTGGA | CGGGACTTACATGACTTCGGT | 595 | 59.8 |
Exon2 | CTGCTGCTCCTGCTCCTGGTA | GGAAGGGAATCTCGTGAGGC | 370 | 59.4 |
Exon3 | AAACCGAAGTCATGTAAGTCC | CAATAATCCCAATTTCTAGTCC | 336 | 54.0 |
Exon4a | TTAGAGGTCCCTGCCACA | ATTCCCCTGCCTTCACAA | 629 | 52.2 |
Exon4b | TGCAACATGCCAGCCACT | ATAGCAAATTGAACACCC | 460 | 50.0 |