Fig. 4

Effects of NDRG2 on ovarian cancer cell apoptosis and cell cycle SKOV3, OVCAR-3, and CAOV3 cells were transfected with vector (negative control), NDRG2 OE, si-NC (negative control), or si-NDRG3. a Cell apoptosis was examined by Flow cytometry. The Quadrant 1 (Q1) of the flow diagram represented as dead cells; the Quadrant 2 (Q2) represented as non-viable apoptotic cells; the Quadrant 3 (Q3) represented as viable apoptotic cells; the Quadrant 4 (Q4) represented as viable non-apoptotic cells. The percentage of cell apoptosis is the sum of Q2 and Q3 in the flow diagram. b Western blot assay was adopted to examine apoptosis-related protein BAX, BCL2 and cleaved caspase3/caspase3. and c Cell cycle was detected by Flow cytometry. d Cell cycle marker protein cyclin B1 and cyclin A2 had been examined by western blot assay. **P < 0.01, compared to Vector group; ##P < 0.01, compared to si-NC group