Fig. 2

MiR-490-3p interacted with TONSL-AS1 but did not regulate its expression. RNA interaction prediction performed using IntaRNA (http://rna.informatik.uni-freiburg.de/IntaRNA/Input.jsp) showed that miR-490-3p can bind TONSL-AS1 (a). Dual luciferase reporter assay was performed by transfecting TONSL-AS1 + miRNA NC (NC group) or TONSL-AS1 + miR-490-3p (miR-490-3p) into OVCAR3 cells (b). To analyze the relationship between them, OVCAR3 cells were transfected with TONSL-AS1 expression and miR-490-3p mimic. Overexpression of TONSL-AS1 and miR-490-3p was confirmed by qPCR (c). The effects of TONSL-AS1 overexpression on miR-490-3p (d) and the effects of overexpression of miR-490-3p on TONSL-AS1 (e) were also analyzed by qPCR. Experiments were repeated 3 times and data were expressed as mean values, *, p < 0.05