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Fig. 3 | Journal of Ovarian Research

Fig. 3

From: Activated CAMKKβ-AMPK signaling promotes autophagy in a spheroid model of ovarian tumour metastasis

Fig. 3

Pharmacologic inhibition of AMPK using Compound C increases LC3-II and blocks autophagic flux in spheroids. a iOvCa147-MA and OVCAR8 cells were seeded into 6-well ULA plates to form spheroids for 24 h prior to treatment with Compound C at the indicated concentrations; DMSO was the vehicle control. Protein lysates were isolated at 24 h post-treatment. Immunoblot analysis was performed for p-AMPK (T172), AMPK, p62, and LC3B; tubulin served as a loading control. b Densitometric analysis for p62/tubulin and LC3-II:I ratio from the immunoblots were tested by one-way ANOVA followed by Dunnett’s multiple comparison test. Asterisks indicate significant differences relative to control (*, p < 0.05; **, p < 0.01). c OVCAR8 mCherry-eGFP-LC3B cells were seeded into 24-well ULA plates, cultured for 24 h, then treated with 10 μM Compound C, or DMSO as a control, for an additional 24 h. Phase contrast and fluorescence images were captured. Scale bar = 200 μm

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